FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59899
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19783.3022254127781765No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3630.6060201338920516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.4791398854738811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.39232708392460647No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT1600.2671163124593065No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1530.25542997378921184No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.25376049683634117No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1320.22037095777892785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.21703200387318652No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1270.21202357301457453No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1210.20200671129735054No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1150.19198984958012655No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1140.19032037262725587No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1100.18364246481577323No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC1030.17195612614567857No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG1000.16694769528706657No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG980.16360874138132525No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA930.1552613566169719No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA920.15359187966410123No Hit
GTACATGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTC890.14858344880548924No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT860.14357501794687724No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCTG790.13188867927678258No Hit
CCTCTTGACTGTGGCATAGAGTTATTTCTCCAAACAAGCACGCGGCATTT780.1302192023239119No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT780.1302192023239119No Hit
GTATTAATATTTTAAATGAATCACTCCTTTTCCTTTCCTCCAATGTCCCA770.12854972537104126No Hit
GAGTAGAAGCAAACAGCAAACTCAGCAGAAGTCTGTGTAGATCCTCCGAA770.12854972537104126No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTT740.12354129451242926No Hit
GTACATGGGGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAA710.11853286365381727No Hit
GTACATGGGCTGGGCATGGTGGCAGGCACCTGTAGTCCCAGCTACTCAGG700.1168633867009466No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA690.11519390974807592No Hit
GTGATAGGGACTTCACATGTGTTCCCTAATGTATTCCTCACACCTCGACA680.11352443279520526No Hit
CCTTATGAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTG680.11352443279520526No Hit
GTATAAGTAACATGAAAACATTCTCCTCTGCATAAGCCTGCGTCAGATTA640.1068465249837226No Hit
ACCTAGATGTTTTGTTTTGTTTTTAGATGGAGTCTCACTCTGTCACCCAG630.10517704803085194No Hit
GTCCAGGAGGATGGCTTGGGAAATGCCGCGTGCTTGTTTGGAGAAATAAC620.10350757107798127No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)