FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88482
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT31573.567957324653602No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT12751.4409710449582966RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT7990.9030085215071991RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.8171153454939987No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG6270.7086187021089035RNA PCR Primer, Index 46 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.7029678352659298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.5763884179833186No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT4070.4599805610180602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.4373770936461653No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT3410.38538911869080716RNA PCR Primer, Index 46 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.2927149024660383No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2330.263330394882575No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA2180.24637779435365384RNA PCR Primer, Index 46 (96% over 28bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2180.24637779435365384No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG1910.21586311340159578TruSeq Adapter, Index 4 (95% over 23bp)
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1760.1989105128726747No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.18760877918672725No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1610.18195791234375353No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1530.17291652539499558No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGT1440.1627449650776429No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1420.1604846183404534No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1400.15822427160326394No Hit
GTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATC1400.15822427160326394No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTT1380.15596392486607447No Hit
GTACAAAGAGTTGTCATGGCAATGAGTTTGGCTGATGCAAAGGTCATTGT1330.15031305802310074No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1320.149182884654506No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCT1290.14579236454872177Illumina PCR Primer Index 8 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1280.14466219118012702No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1230.1390113243371533No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT1220.13788115096855857No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1140.12883976401980063No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT1130.1277095906512059No Hit
ACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1100.12431907054542167No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT1090.12318889717682692No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG1070.12092855043963743No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC1060.1197983770710427TruSeq Adapter, Index 18 (95% over 24bp)
GTTCTACTCCATGCTGAAGGAGCTGAAAGCTGCCTTCTTCATATTTTGCA1050.11866820370244795No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.11527768359666374No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.11414751022806899Clontech SMART CDS Primer II A (100% over 21bp)
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1010.11414751022806899No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT990.1118871634908795No Hit
GGTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT980.11075699012228475No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC970.10962681675369002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.10962681675369002No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC970.10962681675369002No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT970.10962681675369002No Hit
GTACATGGGAACCAATGAAGTGTTTGTCAGTATGCCCCAAAGCTTGCTCT950.10736647001650054No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT930.10510612327931104No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT920.1039759499107163No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC920.1039759499107163No Hit
GTACATGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTC920.1039759499107163No Hit
GTACATGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATT920.1039759499107163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC910.10284577654212156No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC900.10171560317352682No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)