FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88482
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10161.1482561424922584No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.3673063447932913No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2480.28028299541149615No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2010.22716484708754323No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1850.20908207319002736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.20117085960986414No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1590.17969756560656405No Hit
GTACAAAGAGTTGTCATGGCAATGAGTTTGGCTGATGCAAAGGTCATTGT1550.17517687213218508No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1520.17178635202640086No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1500.16952600528921138No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1490.16839583192061663No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1490.16839583192061663No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT1430.1616147917090482No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1360.15370357812888497No Hit
GTTCTACTCCATGCTGAAGGAGCTGAAAGCTGCCTTCTTCATATTTTGCA1360.15370357812888497No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1360.15370357812888497No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT1340.1514432313916955No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.14805271128591127No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT1280.14466219118012702No Hit
GTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATC1270.1435320178115323No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA1210.13675097759996382No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1200.1356208042313691No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG1170.13223028412558485No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1150.12996993738839538No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT1140.12883976401980063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.1277095906512059No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1100.12431907054542167No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC1090.12318889717682692No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG1050.11866820370244795No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1040.11753803033385322No Hit
ACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1010.11414751022806899No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT990.1118871634908795No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA990.1118871634908795No Hit
GTACATGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATT990.1118871634908795No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG980.11075699012228475No Hit
GTACATGGGAACCAATGAAGTGTTTGTCAGTATGCCCCAAAGCTTGCTCT980.11075699012228475No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG960.10849664338509526No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA960.10849664338509526No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG950.10736647001650054No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG930.10510612327931104No Hit
GTACATGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTC920.1039759499107163No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT920.1039759499107163No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT910.10284577654212156No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC910.10284577654212156No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT900.10171560317352682No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT900.10171560317352682No Hit
GAATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGC900.10171560317352682No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT900.10171560317352682No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)