FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65422
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT43836.699581180642597No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT17142.6199137904680385RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT11971.8296597474855552RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG9991.5270092629390724RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT6180.9446363608572039No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT4570.6985417749380942RNA PCR Primer, Index 17 (96% over 26bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG3150.4814894072330409TruSeq Adapter, Index 12 (95% over 23bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA2970.45397572681972426RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTT2340.3576778453731161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.3485066185686772No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAGCTCGTATGCCGT2010.30723609794870227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.2858365687383449No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC1660.25373727492280884TruSeq Adapter, Index 25 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTTCT1510.23080920791171164RNA PCR Primer, Index 19 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC1350.20635260309987466No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC1260.19259576289321637No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT1220.1864816116902571No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGT1200.18342453608877748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.14215401546880255No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA790.12075448625844516No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC730.1115832594540063No Hit
TCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTTCTGCTTG700.10699764605178687RNA PCR Primer, Index 19 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)