FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19140
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT363618.996865203761757No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT14157.39289446185998RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT10105.276907001044933RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG8724.555903866248694RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT4402.2988505747126435No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT3701.9331243469174502RNA PCR Primer, Index 33 (96% over 27bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA3041.5882967607105538RNA PCR Primer, Index 33 (96% over 29bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG2741.4315569487983282RNA PCR Primer, Index 15 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT1650.8620689655172413No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT1560.8150470219435737No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1480.7732497387669801No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1470.768025078369906RNA PCR Primer, Index 15 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT1260.658307210031348RNA PCR Primer, Index 33 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCT1130.5903866248693835Illumina PCR Primer Index 4 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC1080.5642633228840125No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT860.44932079414838033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.3918495297805642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.3918495297805642No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC680.3552769070010449No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTG640.3343782654127482Illumina PCR Primer Index 4 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTT580.30303030303030304No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC530.2769070010449321No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC500.2612330198537095No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT470.24555903866248693No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG400.20898641588296762No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCT390.20376175548589343Illumina PCR Primer Index 4 (95% over 21bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC370.19331243469174503No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT350.18286311389759666No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTCTT350.18286311389759666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.17763845350052246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.17763845350052246No Hit
CTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTGA320.1671891327063741Illumina PCR Primer Index 4 (96% over 26bp)
TCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCTGCTTG300.1567398119122257Illumina PCR Primer Index 4 (96% over 26bp)
CATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCT300.1567398119122257Illumina PCR Primer Index 4 (95% over 24bp)
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT300.1567398119122257RNA PCR Primer, Index 15 (96% over 27bp)
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG260.13584117032392895No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.13061650992685475No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTG250.13061650992685475RNA PCR Primer, Index 48 (95% over 24bp)
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC220.11494252873563218No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTT220.11494252873563218No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCT220.11494252873563218No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA210.109717868338558No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCT210.109717868338558Illumina PCR Primer Index 4 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCCT200.10449320794148381No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA200.10449320794148381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)