FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22524
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT457420.307227845853312No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT19428.621914402415202TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG13265.887053809270112TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT12285.451962351269756RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT6522.8946901083288936No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT4892.1710175812466703TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA4582.0333866098383946RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG3131.3896288403480732TruSeq Adapter, Index 3 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT2461.0921683537559936No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT2431.0788492274906767No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1980.8790623335109218No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT1710.7591901971230687Illumina PCR Primer Index 8 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT1700.754750488367963No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1650.7325519445924348Illumina PCR Primer Index 12 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC1450.6437577694903215No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT1310.5816018469188421No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG1180.5238856331024685Illumina PCR Primer Index 8 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.3951340792044042No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT880.3906943704492985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.38181495293908724No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG830.36849582667377023No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT770.3418575741431362No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT750.33297815663292485Illumina PCR Primer Index 8 (95% over 21bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG720.31965903036760784No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC640.28414136032676257No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC600.26638252530633993No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT570.25306339904102293Illumina PCR Primer Index 8 (95% over 24bp)
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA530.23530456402060027Illumina PCR Primer Index 8 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.2175457290001776No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT420.18646776771443793TruSeq Adapter, Index 3 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT410.18202805895933227No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG370.1642692239389096No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG330.14651038891848694Illumina PCR Primer Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGTCTT320.14207068016338129No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGT320.14207068016338129No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTTCT300.13319126265316997Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11987213638785296No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG270.11987213638785296Illumina PCR Primer Index 8 (96% over 26bp)
GTACTTGTTCGCTATCGGTCTCTCGTCGATATTTAGCTTTAGAAGAAATT270.11987213638785296No Hit
GTACATGGGAGAGTCGAGTAGTTTGGGATTGCTGCTCAAAGTGGGTGGTA260.11543242763274728No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTC260.11543242763274728No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCT250.11099271887764163Illumina PCR Primer Index 8 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCC230.1021133013674303No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCCTG230.1021133013674303Illumina PCR Primer Index 8 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)