FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776275
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT54050.6962738720170043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21770.2804418537245177No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT19240.24785031077904093RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.2268525973398602No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT14130.18202312324884867RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG13060.16823934816914107RNA PCR Primer, Index 17 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.10923963801487875No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT8430.10859553637564008No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)