FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20870
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT548526.28174413033062No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT20699.913751796837566RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT15247.302347867752755RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG15057.211308097747963RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT6162.95160517489219RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA5662.7120268327743173RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT4161.9932918064206995No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG3571.71058936272161RNA PCR Primer, Index 26 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT2381.1403929084810733No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2171.039770004791567RNA PCR Primer, Index 26 (95% over 22bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC1990.9535218016291327RNA PCR Primer, Index 26 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC1630.7810253953042645No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT1590.7618591279348347RNA PCR Primer, Index 26 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT1460.6995687589841878RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT1270.6085289889793962No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG1120.5366554863440345No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT960.4599904168663153RNA PCR Primer, Index 26 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC880.42165788212745564No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT700.33540967896502155RNA PCR Primer, Index 26 (95% over 22bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA690.3306181121226641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.3162434115955918No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT650.3114518447532343RNA PCR Primer, Index 26 (96% over 30bp)
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC600.287494010541447RNA PCR Primer, Index 26 (95% over 22bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG580.27791087685673216No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG540.2587446094873024RNA PCR Primer, Index 26 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.23478677527551509No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCT450.21562050790608528RNA PCR Primer, Index 26 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATATCGTATGCCGT400.19166267369429804No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTT350.16770483948251078RNA PCR Primer, Index 26 (95% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCTTG330.1581217057977959RNA PCR Primer, Index 26 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTTAGCATCTCGTATGCCGT330.1581217057977959No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCCT300.1437470052707235RNA PCR Primer, Index 26 (95% over 22bp)
CATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCT300.1437470052707235RNA PCR Primer, Index 26 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTTCT280.13416387158600862RNA PCR Primer, Index 26 (95% over 24bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA270.1293723047436512RNA PCR Primer, Index 26 (96% over 31bp)
CATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCT260.12458073790129372RNA PCR Primer, Index 26 (96% over 27bp)
CTCGGAGATGTGTATAAGAGACAGATCTCCGAGCCCACGAGACTCCTGAG260.12458073790129372No Hit
CTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTGA240.11499760421657883RNA PCR Primer, Index 26 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)