FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16594
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT396923.918283717006148No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14358.647703989393756TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT9955.9961431842834765RNA PCR Primer, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG8094.875256116668676TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGT5033.0312161022056165No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT4252.561166686754248TruSeq Adapter, Index 1 (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG2491.5005423647101361TruSeq Adapter, Index 1 (95% over 24bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA2481.4945160901530674RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT1781.07267687115825No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTT1560.940098830902736No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC1230.7412317705194649Illumina PCR Primer Index 5 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1200.7231529468482584No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC1130.6809690249487766No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT1040.6267325539351573No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCT1030.6207062793780885RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGT990.5966011811498132No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT950.5724960829215379No Hit
ATATAACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCCCACGTT940.5664698083644691No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT750.45197059178016147No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCTT710.4278654935518862No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC650.3917078462094733No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC610.367602747981198No Hit
GAACTATCATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCATCACTT590.35555019886706035No Hit
GTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGACTGCA580.3495239243099915No Hit
ACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCAT540.3254188260817163No Hit
GTTTTATAAGAATCCCACGTTTCCATCACTTATTTTTCATATACAAATTC510.3073400024105098No Hit
TCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCTGCTTG500.301313727853441TruSeq Adapter, Index 5 (96% over 26bp)
ATGTAAATATAACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCC500.301313727853441No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCTTCT440.26515608051102807RNA PCR Primer, Index 5 (95% over 21bp)
GTACATGGGGTAACACCACTTTTTACTCATTCTTTTATTTGTTATTATTC430.25912980595395924No Hit
GGGTAACACCACTTTTTACTCATTCTTTTATTTGTTATTATTCGAAATCA420.25310353139689046No Hit
ATCATTCACAATGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCAT400.2410509822827528No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCA370.22297215861154634No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT360.2169458840544775TruSeq Adapter, Index 1 (96% over 27bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAGGCAT350.2109196094974087No Hit
GTTCTGCAGTCTGTAAATGCAAAGACATTTTACATCATGAAACATGCTAT350.2109196094974087No Hit
CATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCTGCT330.19886706038327104TruSeq Adapter, Index 5 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.19886706038327104No Hit
ATGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGACTG320.19284078582620226No Hit
GTATCAACGCAGAGTACATGGGGTAACACCACTTTTTACTCATTCTTTTA320.19284078582620226No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGTCTT300.1807882367120646No Hit
TTTTATAAGTGACAGTTTGGACACTTTTAAGGTAAATAAATGCATGACTT290.17476196215499576No Hit
TATCAACGCAGAGTACATGGGGTAACACCACTTTTTACTCATTCTTTTAT270.16270941304085815No Hit
TTCTTTTATTTGTTATTATTCGAAATCATTCACAATGTAAATAGCATGTT270.16270941304085815No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAAATCGTATGCCGT260.1566831384837893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.1566831384837893No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCTG250.1506568639267205TruSeq Adapter, Index 5 (95% over 22bp)
GAATTATGTAAATATAACACAGAACTATCATTTCCACTTTGTTTTATAAG240.1446305893696517No Hit
CTTTTATTTGTTATTATTCGAAATCATTCACAATGTAAATAGCATGTTTC230.13860431481258287No Hit
CTTTTTACTCATTCTTTTATTTGTTATTATTCGAAATCATTCACAATGTA230.13860431481258287No Hit
GGTATCAACGCAGAGTACATGGGGTAACACCACTTTTTACTCATTCTTTT220.13257804025551403No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGTCTTCT220.13257804025551403RNA PCR Primer, Index 5 (95% over 21bp)
GTCTTTGCATTTACAGACTGCAGAACAAGGTGAATTTGTATATGAAAAAT220.13257804025551403No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGTCTT220.13257804025551403No Hit
GTAATGAATTATGTAAATATAACACAGAACTATCATTTCCACTTTGTTTT210.12655176569844523No Hit
CTGCAGAACAAGGTGAATTTGTATATGAAAAATAAGTGATGGAAACGTGG210.12655176569844523No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGTCTTCT210.12655176569844523RNA PCR Primer, Index 5 (95% over 21bp)
GTTTCATGATGTAAAATGTCTTTGCATTTACAGACTGCAGAACAAGGTGA210.12655176569844523No Hit
CATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCATCACTTATTTTTC210.12655176569844523No Hit
ATTCGAAATCATTCACAATGTAAATAGCATGTTTCATGATGTAAAATGTC200.1205254911413764No Hit
GAGTACATGGGGTAACACCACTTTTTACTCATTCTTTTATTTGTTATTAT200.1205254911413764No Hit
CTTATTTTTCATATACAAATTCACCTTGTTCTGCAGTCTGTAAATGCAAA200.1205254911413764No Hit
CTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCTGCTTGA190.11449921658430759RNA PCR Primer, Index 5 (96% over 26bp)
AAATATAACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCCCACG190.11449921658430759No Hit
CAATGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGAC180.10847294202723876No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTC180.10847294202723876No Hit
CTTTTAAGGTAAATAAATGCATGACTTAACTAGACAATTTTGTAATGAAT180.10847294202723876No Hit
CATTTACAGACTGCAGAACAAGGTGAATTTGTATATGAAAAATAAGTGAT170.10244666747016994No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCC170.10244666747016994No Hit
CATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCTTCTGCT170.10244666747016994TruSeq Adapter, Index 5 (95% over 24bp)
TTTACAGACTGCAGAACAAGGTGAATTTGTATATGAAAAATAAGTGATGG170.10244666747016994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)