FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16594
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCCCACGTT1130.6809690249487766No Hit
GTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGACTGCA690.41581294443774863No Hit
ACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCAT690.41581294443774863No Hit
GAACTATCATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCATCACTT660.3977341207665421No Hit
GTACATGGGGTAACACCACTTTTTACTCATTCTTTTATTTGTTATTATTC620.37362902253826685No Hit
GTTTTATAAGAATCCCACGTTTCCATCACTTATTTTTCATATACAAATTC620.37362902253826685No Hit
ATGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGACTG500.301313727853441No Hit
GGGTAACACCACTTTTTACTCATTCTTTTATTTGTTATTATTCGAAATCA470.28323490418223457No Hit
GTTCTGCAGTCTGTAAATGCAAAGACATTTTACATCATGAAACATGCTAT450.2711823550680969No Hit
ATCATTCACAATGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCAT430.25912980595395924No Hit
ATGTAAATATAACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCC380.22899843316861518No Hit
TTTACAGACTGCAGAACAAGGTGAATTTGTATATGAAAAATAAGTGATGG360.2169458840544775No Hit
GTATCAACGCAGAGTACATGGGGTAACACCACTTTTTACTCATTCTTTTA340.20489333494033987No Hit
CTGCAGAACAAGGTGAATTTGTATATGAAAAATAAGTGATGGAAACGTGG300.1807882367120646No Hit
GAATTATGTAAATATAACACAGAACTATCATTTCCACTTTGTTTTATAAG270.16270941304085815No Hit
CTTTTTACTCATTCTTTTATTTGTTATTATTCGAAATCATTCACAATGTA270.16270941304085815No Hit
CTTTTAAGGTAAATAAATGCATGACTTAACTAGACAATTTTGTAATGAAT270.16270941304085815No Hit
TTTTATAAGTGACAGTTTGGACACTTTTAAGGTAAATAAATGCATGACTT270.16270941304085815No Hit
CTTTTATTTGTTATTATTCGAAATCATTCACAATGTAAATAGCATGTTTC240.1446305893696517No Hit
ATGTAAAATGTCTTTGCATTTACAGACTGCAGAACAAGGTGAATTTGTAT230.13860431481258287No Hit
GTAAAATGTCTTTGCATTTACAGACTGCAGAACAAGGTGAATTTGTATAT220.13257804025551403No Hit
TGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGACTGC220.13257804025551403No Hit
CTGTAAATGCAAAGACATTTTACATCATGAAACATGCTATTTACATTGTG210.12655176569844523No Hit
TATCAACGCAGAGTACATGGGGTAACACCACTTTTTACTCATTCTTTTAT210.12655176569844523No Hit
GGTATCAACGCAGAGTACATGGGGTAACACCACTTTTTACTCATTCTTTT200.1205254911413764No Hit
CAATGTAAATAGCATGTTTCATGATGTAAAATGTCTTTGCATTTACAGAC200.1205254911413764No Hit
TTCTTTTATTTGTTATTATTCGAAATCATTCACAATGTAAATAGCATGTT200.1205254911413764No Hit
CATTTACAGACTGCAGAACAAGGTGAATTTGTATATGAAAAATAAGTGAT200.1205254911413764No Hit
GAGTACATGGGGTAACACCACTTTTTACTCATTCTTTTATTTGTTATTAT200.1205254911413764No Hit
CATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCATCACTTATTTTTC190.11449921658430759No Hit
GTCTTTGCATTTACAGACTGCAGAACAAGGTGAATTTGTATATGAAAAAT180.10847294202723876No Hit
ATTCGAAATCATTCACAATGTAAATAGCATGTTTCATGATGTAAAATGTC180.10847294202723876No Hit
GTTTCATGATGTAAAATGTCTTTGCATTTACAGACTGCAGAACAAGGTGA180.10847294202723876No Hit
CAAATTCACCTTGTTCTGCAGTCTGTAAATGCAAAGACATTTTACATCAT170.10244666747016994No Hit
AAATATAACACAGAACTATCATTTCCACTTTGTTTTATAAGAATCCCACG170.10244666747016994No Hit
ATCATTTCCACTTTGTTTTATAAGAATCCCACGTTTCCATCACTTATTTT170.10244666747016994No Hit
ATTTGTTATTATTCGAAATCATTCACAATGTAAATAGCATGTTTCATGAT170.10244666747016994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)