FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418841
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT55291.3200713397208008No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG19110.4562590577331255RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT18980.45315525461929473RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.445276369791878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.35359480089103024No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT13110.3130066063255508RNA PCR Primer, Index 39 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.19052576037207436No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA6970.16641159771846598RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT5310.12677841949570362RNA PCR Primer, Index 39 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)