FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745207
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT187282.5131272250529046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85051.1412936271398415No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT84451.133242172980125RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT64050.8594927315497572RNA PCR Primer, Index 23 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59190.794275952856052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54340.7291933650650088No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG23970.32165559368068203RNA PCR Primer, Index 23 (96% over 30bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG18180.2439590610394159Illumina PCR Primer Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT18100.24288553381812034RNA PCR Primer, Index 23 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.21269258071918273No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.17605846429247174No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT12340.16559157388484005No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.1612974649996578No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA11920.15995555597303837No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC10420.13982692057374663No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG9920.1331173754406494No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT9890.13271480273266353No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC9790.13137289370604407No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT9440.12667621211287602No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC9180.12318724864366545No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC9040.12130857600639823No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG8920.11969828517445488No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC8840.11862475795315931No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC8840.11862475795315931No Hit
GTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT8140.10923139476682318No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT8100.10869463115617539RNA PCR Primer, Index 23 (95% over 22bp)
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA8050.10802367664286566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.10721853122689401Clontech SMART CDS Primer II A (100% over 21bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA7960.10681595851890816RNA PCR Primer, Index 23 (96% over 30bp)
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG7930.10641338581092233No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG7850.10533985858962677No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC7850.10533985858962677No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC7620.10225346782840204No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC7560.10144832241243038No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC7480.1003747951911348No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC7460.10010641338581092No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)