FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47491
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1234225.988081952369924No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT46989.89240066538923RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT30816.487545008527931RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10852.284643406119054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9371.9730054115516624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9051.905624223537091No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG7391.5560843107115032RNA PCR Primer, Index 15 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC5821.2254953570150136No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4841.019140468720389RNA PCR Primer, Index 33 (95% over 21bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG3470.7306647575330063RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT3010.6338042997620602RNA PCR Primer, Index 33 (96% over 27bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.5622117874965783Clontech SMART CDS Primer II A (100% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC2230.4695626539765429No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC2190.46114000547472156No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2090.4400833842201681No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.4316607357183466No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1750.36849087195468616RNA PCR Primer, Index 31 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.26320776568191867No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1240.2611021035564633RNA PCR Primer, Index 15 (96% over 25bp)
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1210.2547851171800973No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT1200.25267945505464195No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG1100.2316228338000884RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1040.21898886104735632TruSeq Adapter, Index 3 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.216883198921901No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.2042492261691689No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA960.20214356404371353RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG950.20003790191825818RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG810.1705586321618833TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG810.1705586321618833RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT780.16424164578551725No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT660.13897370028005307No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT630.13265671390368702No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG620.13055105177823165TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG580.12212840327641027RNA PCR Primer, Index 46 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.1158114169000442No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA540.11370575477458887RNA PCR Primer, Index 31 (96% over 29bp)
GCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG530.11160009264913352No Hit
AGCTAGAACTGTGTGTAGCACAAAGTAGATAATTATTTGATTTGTGTTGA510.10738876839822283No Hit
TTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT500.10528310627276748RNA PCR Primer, Index 15 (95% over 21bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA500.10528310627276748RNA PCR Primer, Index 27 (96% over 30bp)
GTTCTAGCTAGTGAGAATGTGAACACAAACAAGTCAGCTTCCTTCTTTTG490.10317744414731211No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCATCTCGTATGCCGTC480.10107178202185677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)