FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148898
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1790812.027025211890018No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT78705.285497454633373TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT47773.208236510900079RNA PCR Primer, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24391.6380340904511812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21361.434539080444331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16881.133661969939153No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG13630.9153917446842805TruSeq Adapter, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG12060.8099504358688497TruSeq Adapter, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT11910.7998764254724711TruSeq Adapter, Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC7580.5090733253636718No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT6910.4640760789265135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.3525903638732555Clontech SMART CDS Primer II A (100% over 21bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA5070.3405015513976011RNA PCR Primer, Index 11 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.3163239264462921No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC4430.29751910703971846RNA PCR Primer, Index 17 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC3680.2471490550578248No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.2323738398098027No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC3420.229687437037435No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2690.18066058644172522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.17797418366935755No Hit
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2560.17192977743153032No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA2310.15513976010423244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.15312495802495668No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2180.14640895109403754Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT2100.14103614554930222No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT1510.10141170465687921No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)