FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487647
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110982.2758265712697914No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14660.3006272980250058No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT13720.2813510592703328Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG11740.2407479180636813No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC7330.1503136490125029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)