FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269009
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60322.242304160827333No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.29664435018902713No Hit
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG7740.28772271559687596No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT5800.21560616931032045Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC3890.1446048273477839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3390.12601808861413558No Hit
ATATTGCCCAGGCAGGTCTCGAACTCCTGGGCTCAAGCTATCCTCCCGCC3190.11858339312067626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2910.1081748194298332Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)