FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298623
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT274029.176118383379713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135094.523764077113953No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT106003.549626117211333RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89703.0037873840929867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79362.6575314024706738No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT67932.2747745485109987RNA PCR Primer, Index 17 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35451.1871155269352995No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31171.0437910006931819No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30451.019680332727218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.6114733292479145Clontech SMART CDS Primer II A (100% over 21bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG15510.5193839724334696TruSeq Adapter, Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC14970.5013009714589968No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.4303084491147701No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.4085418738677195No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT11310.37873840929868097No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC7370.24679947626271254No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG7070.2367533646102276RNA PCR Primer, Index 17 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6790.227376993734575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT6170.20661502965277292RNA PCR Primer, Index 17 (95% over 21bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT6160.20628015926435675RNA PCR Primer, Index 17 (96% over 26bp)
GTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT5490.1838438432404738No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA5330.17848591702581515No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC5130.1717885092574919No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5030.16843980537333025Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.16743519420808176No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.14935219323360893No Hit
GTACATGGGGAGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGTTGGTT4430.14834758206836043No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.1312691922591361No Hit
CTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC3850.12892509954022296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT3630.12155795099506736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3630.12155795099506736No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG3610.12088821021823504RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG3540.1185441174993219RNA PCR Primer, Index 27 (96% over 30bp)
GCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT3110.10414469079742686RNA PCR Primer, Index 17 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)