FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298623
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146204.895805078644311No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17820.5967390321576035No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT7430.2488086985932095Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG7250.24278103160171854No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5690.19054125100879704Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC5530.18518332479413843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC4970.16643058304283326No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4810.16107265682817465Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC4510.15102654517568975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC4470.1496870636220251No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC4350.14566861896103114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC4170.13964095196954018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.1386363408042917No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCC3650.1222276917718997No Hit
GTACATGGGGAGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGTTGGTT3370.11285132089624711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.10447956118584303No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3040.10180059807851372Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)