FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243252
Sequences flagged as poor quality0
Sequence length100
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT3823715.719089668327497No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT152326.261819018959762RNA PCR Primer, Index 26 (100% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152116.25318599641524No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT99374.0850640488053545RNA PCR Primer, Index 26 (100% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94663.891437686021081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94043.86594971469916No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46851.9259862200516336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39531.6250637199283047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37761.552299672767336No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG24190.9944419778665746RNA PCR Primer, Index 26 (96% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG23200.9537434430138292RNA PCR Primer, Index 26 (100% over 29bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.8328811273905251Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC20150.8283590679624423RNA PCR Primer, Index 26 (95% over 22bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT19280.7925936888494236RNA PCR Primer, Index 26 (100% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT17140.7046190781576308RNA PCR Primer, Index 26 (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.6022560965583017No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.5940341703254238No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC11140.45796129117129564No Hit
GTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT9550.3925969776199168RNA PCR Primer, Index 26 (100% over 22bp)
GTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8350.3432654202226498No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC7920.3255882788219624RNA PCR Primer, Index 26 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC7600.31243319684935783No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA7110.29228947757880713RNA PCR Primer, Index 26 (100% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG6440.2647460246986664No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.25529080953085687No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC5230.21500337098975547RNA PCR Primer, Index 26 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5020.20637034844523378No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.20390377057537037No Hit
GCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT4630.19033759229112196RNA PCR Primer, Index 26 (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4600.18910430335619027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.18047128081166855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT4340.1784157992534491No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3790.15580550211303504Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3560.1463502869452255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3330.136895071777416No Hit
GTACATGGGGCAACCGTTCGCTAACTGAAATGATGGCGACTGGAACGCCA3310.13607287915412822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3240.133195204972621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3200.13155081972604543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2910.11962902668837254No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.11798464144179698No Hit
GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT2820.11592915988357752Clontech SMART CDS Primer II A (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2770.11387367832535807No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2720.11181819676713861Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT2660.10935161889727525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2460.1011296926643974No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)