FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601389
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT579479.635527088124325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267894.4545211169476No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT216943.6073157307499804RNA PCR Primer, Index 27 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160572.669985649887178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149772.490401387454709No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT145162.413745512471961RNA PCR Primer, Index 27 (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74181.2334778321519018No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65621.0911406760017226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60761.0103277579071117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.5462354648987594Clontech SMART CDS Primer II A (100% over 21bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG29930.49768120135220295RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC29180.48521007201661487No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24500.40739022496254507No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT23040.38311309318926684RNA PCR Primer, Index 27 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.37529785213896494No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC16560.27536253572978553No Hit
GTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT11450.19039257452331185RNA PCR Primer, Index 27 (95% over 23bp)
GTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11330.18839719382961775No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC10840.18024938933036688RNA PCR Primer, Index 27 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC10830.18008310760589236No Hit
GTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGACTCAC9320.1549745672102416No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.15164893272075144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8460.1406743389054339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT8270.13751498614041827No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.13651729579357122No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8070.1341893516509281Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC7490.12454501163140662No Hit
GTACATGGGTGGTCCGTAAAGAAAATCTCGGCTGGGCATGGTGGCTCACG7320.12171822231533999No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.11772746092795179No Hit
GTCTAGGCTGGCAGCGTGGCCTCCAGCTCTCAGTTCAGGTGTGTGGACAA6520.10841568435737932No Hit
GTCTTATGGAAAGCAAATATACAATGATTTTCAGTAGGCTTCTGGAATAG6440.10708543056158328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6390.10625402193921071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6370.10592145849026172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6300.10475748641894016No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG6160.10242954227629704RNA PCR Primer, Index 27 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)