FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691012
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT576658.345007033162956No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT229143.3160060896192833RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211763.0644909205628847No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT171042.4752102713122204RNA PCR Primer, Index 39 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128391.85799957164275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114961.6636469410082604No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG89181.2905709307508406RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT57360.8300868870584013RNA PCR Primer, Index 39 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.7303780542161351No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46470.672491939358506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.6652561750013024No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG43100.6237228875909536Illumina PCR Primer Index 11 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC31380.45411657105810027No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA27360.39594102562618305RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.36612967647450406Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT24670.35701261338442747RNA PCR Primer, Index 39 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC19480.2819053793566537Illumina PCR Primer Index 11 (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.27611676787089084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.27105173282084827No Hit
GTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT14650.21200789566606656RNA PCR Primer, Index 39 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA14290.20679814532887997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC13510.1955103529316423No Hit
GTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13050.18885344972301496No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG11810.17090875411714992RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG11700.16931688595856512RNA PCR Primer, Index 27 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11330.16396242033423442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC10730.15527950310559005RNA PCR Primer, Index 39 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10430.15093804449126788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9650.1396502520940302Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG9530.13791366864830132RNA PCR Primer, Index 23 (96% over 30bp)
GTATATACATTATCCTCCCAAATAAGAAACAATTTGATTCCAAAGTTTAA8800.12734945268678402No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG8180.11837710488385152TruSeq Adapter, Index 10 (96% over 28bp)
GTTATATACCCAGTGCTTTAGTTGCTGTTTTTCTCTCTTGAGGGTGGAGA8130.11765352844813114No Hit
GCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT8120.11750881316098707RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG7540.10911532650663086TruSeq Adapter, Index 3 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC7460.10795760420947828No Hit
TTCTAAGAAGGGTCATTTTTTCCCCCCAGTACTGGGAAGGTATGCATTTA7200.10419500674373237No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG7180.10390557616944424TruSeq Adapter, Index 1 (96% over 28bp)
GTATAATGAATGGTAAATGCACCGGTTTGGATTCAGGCACAGCCCCAGTC7180.10390557616944424No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.10130070100085092No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)