FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691012
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209263.0283120987768664No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22650.3277801253813248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.20896887463604108No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT13200.19102417903017602Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG13090.18943231087159124No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11550.16714615665140403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9030.13067790429109769Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC8970.12980961256823326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.12619173038963144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC8330.12054783419101259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC8130.11765352844813114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC8090.11707466729955485No Hit
GTTATATACCCAGTGCTTTAGTTGCTGTTTTTCTCTCTTGAGGGTGGAGA8040.1163510908638345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC7750.11215434753665639No Hit
GTATATACATTATCCTCCCAAATAAGAAACAATTTGATTCCAAAGTTTAA7570.10954947236806306No Hit
GTACATGGGAAGGGTTCATTTTGGAAACTACATTTTAAACTTTGGAATCA7480.10824703478376642No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC7460.10795760420947828No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)