FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545262
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT5538110.156768672674787No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT240564.4118240405529825RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223274.094728772590058No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT174793.205614915398469RNA PCR Primer, Index 23 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137892.5288760265707126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125002.2924759106631307No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG82121.5060649742692505RNA PCR Primer, Index 23 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68691.2597613624276036No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59601.0930525142041807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54400.9976855163205945No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT46830.8588531751708354RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG44080.8084187051362465Illumina PCR Primer Index 1 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC29870.5478100436120618No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT25590.4693156684309562RNA PCR Primer, Index 23 (95% over 22bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA24920.4570279975498017RNA PCR Primer, Index 23 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.4533600360927407Clontech SMART CDS Primer II A (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.4051263429323885No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18400.3374524540496129No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC18390.3372690559767598Illumina PCR Primer Index 1 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG14530.2664773998554823No Hit
GTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT11980.21971089127795443RNA PCR Primer, Index 23 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC11420.2094405991981836No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC11350.20815681268821226RNA PCR Primer, Index 23 (95% over 21bp)
GTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT10000.18339807285305046No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9030.16560845978630456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGT7770.1425003026068202No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7460.13681496234837565Clontech Universal Primer Mix Long (96% over 26bp)
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC7440.13644816620266956No Hit
GTATTAGGGATAATATTCATTTAGCCTTCTGAGCTTTCTGGGCAGACTTG7390.1355311758384043No Hit
GTATTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCTGAGCTGAA7270.13333039896416768No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6780.1243438933943682No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.11975894157304195No Hit
GCACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT6470.11865855313592366RNA PCR Primer, Index 23 (96% over 25bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.11682457240739315No Hit
ATCCTGAACCATCCAGGCCAAATAAGCGCCGGCTATGCCCCTGTATTGGA6340.11627437818883399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT6100.11187282444036079No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.11095583407609554No Hit
GATATACACCACATATCCCATGGGCAATAAAGCGCATTCAATGTGTTTAT5650.10361991116197351No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)