FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545262
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247314.5356177397287905No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25150.4612461532254219No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT16780.3077419662474187Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG14900.2732631285510452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC9470.1736779749918388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC9270.1700100135347778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.16194049832924357No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC8620.15808913879932948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC8470.15533816770653375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC8310.15240379854088493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC8310.15240379854088493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8060.14781884671955867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7400.13571457391125732Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGT6920.12691146641431092No Hit
GTATTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCTGAGCTGAA6880.12617787412289871No Hit
ATCCTGAACCATCCAGGCCAAATAAGCGCCGGCTATGCCCCTGTATTGGA6420.1177415627716584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCC6360.11664117433454009No Hit
GTATTAGGGATAATATTCATTTAGCCTTCTGAGCTTTCTGGGCAGACTTG6170.11315661095033214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.11205622251321383No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)