FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537721
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT481508.954457795027533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199843.7164254325198383No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT185363.4471408035021875TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT135582.5213819062301823RNA PCR Primer, Index 10 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129602.410171817727037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102211.9007998571750035No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG56561.051846589588281TruSeq Adapter, Index 10 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39960.7431363104658364No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT39580.7360694486545997TruSeq Adapter, Index 10 (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.7115214023629355No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.6600076991599733No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG33900.6304384615813777TruSeq Adapter, Index 10 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.5194143431258962Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC24120.4485597549658652No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT19850.36915054461328456No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA15330.2850920830691009RNA PCR Primer, Index 10 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14960.2782111913055283No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.2780252212578642No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC13060.24287688224934492Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC11090.20624078285951267No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT10870.20214944181090191No Hit
GTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10710.19917392104827594No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT10330.19210705923703927No Hit
GTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT10020.18634198775945146RNA PCR Primer, Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC9450.17574169504259643No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT7500.1394775357480924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6800.1264596324116038No Hit
CTATTAACCTATACCAATTTTAAGTTGTGATTAGGGAATGGGGATGTTTT6740.12534381212561907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT6330.11771904017139001No Hit
GCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT6120.1138136691704434TruSeq Adapter, Index 10 (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6020.11195396869380218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC5990.11139605855080982No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.10804859769285557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5610.10432919673957312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5490.10209755616760366No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.10042382573862654No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)