FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001421431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135943
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189390.6039331709791919No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT189320.6037099526362565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126090.4020800122961419No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT86520.2758978718682068TruSeq Adapter, Index 3 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80340.2561908810204777No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT59960.19120245489155893RNA PCR Primer, Index 3 (95% over 23bp)
ATCTAATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTAC38960.12423695201092622No Hit
TTTCATAACTGTCCTTCCCACGGCTGTCCCAATCAGAAGGCGCGTTCAGG35800.11416023824412624No Hit
TGTATGCACCATTCAACTCCTCGCTTTCCATGTGTGAGGTGATGTCCTCG34470.10991908972835283No Hit
TCCCACGGCTGTCCCAATCAGAAGGCGCGTTCAGGTCCTGGGCAACGGGG31410.10016125930860349No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)