FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126900
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT3161124.91016548463357No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT118459.33412135539795TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT88546.977147360126083RNA PCR Primer, Index 3 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43253.408195429472025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30682.417651694247439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22621.7825059101654845No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG20911.6477541371158395TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC15201.19779353821907No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT12190.9605988967691095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.7580772261623325No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG8420.6635145784081954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.6469661150512215Clontech SMART CDS Primer II A (100% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.475177304964539No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC5850.46099290780141844No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.4578408195429472No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC5330.42001576044129235No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT5280.4160756501182033TruSeq Adapter, Index 3 (96% over 26bp)
GTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT5060.3987391646966115RNA PCR Primer, Index 3 (95% over 21bp)
GTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT4770.375886524822695No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG4570.3601260835303388TruSeq Adapter, Index 3 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.28368794326241137No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC3130.2466509062253743No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2950.23246650906225375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT2820.2222222222222222TruSeq Adapter, Index 3 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.22064617809298662No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC2710.21355397951142632Illumina PCR Primer Index 12 (95% over 24bp)
GCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG2710.21355397951142632No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2470.1946414499605989Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2060.16233254531126873Clontech Universal Primer Mix Long (96% over 26bp)
GATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT2060.16233254531126873No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT1750.13790386130811663No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1720.1355397951142632Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG1710.1347517730496454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1630.12844759653270293No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.11189913317572892No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1370.10795902285263988Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1360.10717100078802205No Hit
GGGTAGAGAAGGAACATATGTCAAAACAATAAAGGCTGTATATGACAAAC1300.1024428684003152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)