FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89505
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT956510.686553823808726TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT908010.144684654488575No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT81079.057594547790625RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG43394.847773867381711TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT37944.238869336908553TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG27853.111558013518798TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18392.054633819339702No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA13851.5473995866152728RNA PCR Primer, Index 11 (96% over 28bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC13141.4680744092508797RNA PCR Primer, Index 17 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11941.3340036869448635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11001.2289816211384839No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC10071.1250768113513212No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG9691.0826210826210827RNA PCR Primer, Index 27 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT8810.984302552930004No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.6804089157030333No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.6234288587229764No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC5420.6055527624155076No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG4540.5072342327244288RNA PCR Primer, Index 31 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA4160.4647785039941903No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.42902631137925257No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA3690.41226747109100054RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG3650.40779844701413326TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG3520.39327411876431484TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG3210.3586391821685939RNA PCR Primer, Index 23 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG2910.32512150159208986TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG2750.30724540528462096RNA PCR Primer, Index 39 (96% over 30bp)
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2720.30389363722697055No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG2290.255851628400648RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT2250.2513826043237808RNA PCR Primer, Index 27 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG2180.2435618121892632RNA PCR Primer, Index 46 (96% over 28bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT2100.23462376403552876TruSeq Adapter, Index 11 (96% over 27bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.2178649237472767Clontech SMART CDS Primer II A (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1830.20445785151667503No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA1820.20334059549745823RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1790.19998882743980784RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA1430.15976761074800291RNA PCR Primer, Index 23 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.1586503547287861No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.15306407463270208No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA1360.15194681861348527RNA PCR Primer, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA1250.13965700240210044RNA PCR Primer, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA1210.13518797832523322RNA PCR Primer, Index 3 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.13183621026758283No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT1170.130718954248366No Hit
TATCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1160.12960169822914921TruSeq Adapter, Index 11 (95% over 21bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA1120.125132674152282No Hit
CTTAACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1090.1217809060946316No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA1080.12066365007541478RNA PCR Primer, Index 39 (96% over 30bp)
TTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1000.11172560192168035No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT930.10390480978716274RNA PCR Primer, Index 31 (96% over 27bp)
CTTATCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC910.10167029774872911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)