FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89505
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27473.0691022847885594No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.324004245572873No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT2190.24467906820847996Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC1110.12401541813306519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)