FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345104
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT69572.0159140433028884No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT61361.778014743381705RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT40941.1863090546617832RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40201.1648662432194354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.8006282164217164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.7061639389865084No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG12380.35873244007603505TruSeq Adapter, Index 12 (95% over 23bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG9560.2770179424173582RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG8160.23645046131021372RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT7630.2210927720339376RNA PCR Primer, Index 17 (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.20312717325791646No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC6670.1932750707033242No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.1906671612035792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.18226389725995643No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT5300.15357689276276137No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG4870.14111688070842413RNA PCR Primer, Index 31 (96% over 29bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA4530.1312647781538319RNA PCR Primer, Index 27 (96% over 30bp)
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT4370.12662849459872966No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.12315128193240298Clontech SMART CDS Primer II A (100% over 21bp)
ATCTAATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTAC4020.11648662432194354No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC3690.10692428948954517No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG3510.10170847049005517TruSeq Adapter, Index 3 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.10054939960127962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)