FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12807
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT8316.488639025532912RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7245.653158428984149No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT5734.474115717966737RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG4343.388771765440775RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG2692.100413836183337RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2491.9442492386975874No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT2151.6787694229718122RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA1991.5538377449832124RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1991.5538377449832124RNA PCR Primer, Index 31 (96% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1931.5069883657374872RNA PCR Primer, Index 26 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1841.4367142968688997No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1771.3820566877488873TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1661.2961661591317248No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1651.2883579292574374TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG1250.9760287342859374RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT1210.9447958147887873RNA PCR Primer, Index 27 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG970.7573982978058874RNA PCR Primer, Index 33 (96% over 29bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA950.7417818380573123RNA PCR Primer, Index 26 (100% over 29bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC860.6715077691887249RNA PCR Primer, Index 26 (96% over 28bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG820.6402748496915749RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA800.6246583899429999RNA PCR Primer, Index 31 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.5778090106972749No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG720.5621925509486999RNA PCR Primer, Index 39 (96% over 30bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA660.515343171702975RNA PCR Primer, Index 46 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.4919184820801124No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG620.4841102522058249TruSeq Adapter, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT620.4841102522058249RNA PCR Primer, Index 26 (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC590.46068556258296245RNA PCR Primer, Index 26 (95% over 22bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA580.4528773327086749RNA PCR Primer, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT520.40602795346294995RNA PCR Primer, Index 31 (96% over 27bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC510.3982197235886624No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA500.39041149371437495RNA PCR Primer, Index 3 (96% over 28bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA470.3669868040915124RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG440.34356211446865TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG440.34356211446865RNA PCR Primer, Index 23 (96% over 30bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG420.327945654720075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.31232919497149997No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA370.28890450534863743RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT350.2732880456000624RNA PCR Primer, Index 46 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.26547981572577495Clontech SMART CDS Primer II A (100% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT330.2576715858514875TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA330.2576715858514875RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA290.22643866635433746RNA PCR Primer, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.21863043648004998No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA270.21082220660576245RNA PCR Primer, Index 27 (100% over 28bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC250.19520574685718747TruSeq Adapter, Index 27 (96% over 25bp)
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC240.18739751698289997RNA PCR Primer, Index 26 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT240.18739751698289997TruSeq Adapter, Index 1 (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.18739751698289997No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.14835636761146248No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT190.14835636761146248RNA PCR Primer, Index 1 (95% over 23bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA190.14835636761146248RNA PCR Primer, Index 23 (96% over 30bp)
GAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA170.13273990786288747TruSeq Adapter, Index 27 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT160.12493167798859998RNA PCR Primer, Index 17 (96% over 26bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT150.11712344811431248RNA PCR Primer, Index 33 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT150.11712344811431248RNA PCR Primer, Index 27 (96% over 25bp)
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC140.10931521824002499TruSeq Adapter, Index 18 (95% over 24bp)
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA140.10931521824002499RNA PCR Primer, Index 38 (96% over 28bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA140.10931521824002499RNA PCR Primer, Index 11 (96% over 28bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10150698836573749No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA130.10150698836573749TruSeq Adapter, Index 3 (96% over 28bp)
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT130.10150698836573749RNA PCR Primer, Index 27 (100% over 27bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT130.10150698836573749RNA PCR Primer, Index 26 (96% over 30bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT130.10150698836573749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)