FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12807
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4303.3575388459436244No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.34356211446865No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT190.14835636761146248Illumina RNA PCR Primer (96% over 25bp)
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT170.13273990786288747Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG140.10931521824002499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)