FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432654
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7457817.237330522773394No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT4871811.260268020173164RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT336517.777808595320973RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108962.5184096298658973No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG87492.0221701405742234RNA PCR Primer, Index 27 (100% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70031.6186144124404258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68301.5786286501453817No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC51791.1970304215377645No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT44861.0368562407836286RNA PCR Primer, Index 27 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38760.8958659806681553No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34060.7872341409070527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24500.5662723562014913No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC24290.5614185931483356RNA PCR Primer, Index 27 (95% over 21bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG22820.5274422517762462RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT21530.49762627873543297RNA PCR Primer, Index 27 (96% over 28bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC16130.37281522879714507No Hit
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC15200.3513199924188844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC13370.30902291438424234No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.2542447313557716Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.24800417885885717No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.2327494949775109No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC8280.19137694323870807TruSeq Adapter, Index 27 (96% over 25bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA7600.1756599962094422RNA PCR Primer, Index 27 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4590.1060893924475447Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)