FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097000
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT765476.977848678213309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337743.078760255241568No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT304302.773928896991796RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203001.8505013673655424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193261.7617137648131265No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT192381.7536918869644484RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG98540.8982680036463081RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT72800.6636280765724704RNA PCR Primer, Index 39 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60760.5538742023701003No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG57200.5214220601640839Illumina PCR Primer Index 11 (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.4750227894257064No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.4681859617137648No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC38040.34676390154968095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.33044667274384687Clontech SMART CDS Primer II A (100% over 21bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA33970.3096627164995442RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG31320.2855059252506837RNA PCR Primer, Index 27 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT30800.2807657247037375RNA PCR Primer, Index 39 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.2625341841385597No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC23470.21394712853236098Illumina PCR Primer Index 11 (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.20957155879671835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18500.16864175022789427No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC16020.14603463992707383No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG15950.1453965360072926RNA PCR Primer, Index 31 (96% over 29bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA14420.13144940747493164RNA PCR Primer, Index 27 (96% over 30bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC14300.13035551504102097RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12700.11577028258887875No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA12160.11084776663628076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT11840.10793072014585234No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11740.10701914311759345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG11550.10528714676390155TruSeq Adapter, Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)