FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589208
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT6455010.955384176725365No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT281534.77810891909139RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207543.522355433055899No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT187233.177655428982634RNA PCR Primer, Index 23 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141432.400340796458975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123992.1043502464324995No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG78241.3278842106692372RNA PCR Primer, Index 23 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67531.1461147845921984No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60031.0188252705326473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57650.9784320647377496No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG54210.9200486076224356Illumina PCR Primer Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT53140.9018886369499397RNA PCR Primer, Index 23 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC32710.5551520006517223No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT27720.47046204396410096RNA PCR Primer, Index 23 (95% over 22bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA27450.4658796214579571RNA PCR Primer, Index 23 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.37626780356003314No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.35607120066258435Clontech SMART CDS Primer II A (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.35437400714179035No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC18560.3149991174593692Illumina PCR Primer Index 1 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC12920.21927740288658673RNA PCR Primer, Index 23 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC12780.21690133195747513No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG11900.2019660289744878No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG10410.17667784551465696RNA PCR Primer, Index 27 (96% over 30bp)
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC9000.15274741687146134No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.13933958805718863No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.11693663358270763No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.11557887876607242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.11456056265359602No Hit
GTGTTAAGCAGTGTTTATTTGGTAAGCTGAATCCAAGAAGATGTGTTAGG6450.10946898209121397No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT6280.10658375310586413No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)