FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073252
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT739696.892043993395773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394973.6801235870047293No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT271842.532862738667154RNA PCR Primer, Index 31 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236292.201626458650904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213001.9846224372281625No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT150571.4029323961194575RNA PCR Primer, Index 31 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65030.6059154793096122No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.5565328552846862No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58630.5462836314304562No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.36580411683369796Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC34130.31800546376806194No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.28716461744306093No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG30330.282599054089813RNA PCR Primer, Index 38 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT28180.262566480192909RNA PCR Primer, Index 31 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.2224081576367899No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19650.18308840794147133Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC17550.16352170785612327No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17210.16035376593754308Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14770.13761912393361483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12700.11833194813520032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT12090.11264828763421825No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC11700.10901447190408219No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG11300.10528748141163492RNA PCR Primer, Index 27 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10870.10128096663225411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)