FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377671
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT4804112.720330658165441No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT179004.73957492102915RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT102202.7060589772579835RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83512.211183808129298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57841.5314916951526594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54381.4398775653942189No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG25630.6786329900892576RNA PCR Primer, Index 15 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC22840.6047591686944457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.4726335884936889No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT17650.46733797405678484RNA PCR Primer, Index 33 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.4355642874353604Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.40696796947607844No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.367515641921143No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT12430.3291224372535884RNA PCR Primer, Index 33 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC11000.29125879402972427No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG10970.2904644518641887RNA PCR Primer, Index 33 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.23141835089270818No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC7380.19540817272176045No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC7250.19196602333777282No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.14509983557117173No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC5450.14430549340563611No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC4760.12603562359831705RNA PCR Primer, Index 15 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG4310.11412049111528288RNA PCR Primer, Index 27 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)