FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15910
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT8335.235700817096165RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7924.978001257071025No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT6123.8466373350094276RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2611.640477686989315No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG1931.213073538654934RNA PCR Primer, Index 46 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1841.156505342551854No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG1821.1439346323067252TruSeq Adapter, Index 4 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1771.1125078566939033RNA PCR Primer, Index 46 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1741.09365179132621No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.8045254556882464No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT930.5845380263984915No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG870.546825895663105TruSeq Adapter, Index 1 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC850.5342551854179761No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.5153991200502828No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG810.5091137649277184RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG730.45883092394720304RNA PCR Primer, Index 27 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.44626021370207414No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG670.42111879321181644TruSeq Adapter, Index 3 (96% over 28bp)
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC640.4022627278441232TruSeq Adapter, Index 18 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG520.32683846637335007No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.30169704588309243Clontech SMART CDS Primer II A (100% over 21bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG460.2891263356379635RNA PCR Primer, Index 26 (100% over 29bp)
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC420.26398491514770583No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA380.23884349465744817RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA370.23255813953488372RNA PCR Primer, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG360.22627278441231927RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG350.21998742928975487RNA PCR Primer, Index 17 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.20741671904462602No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG300.18856065367693275TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG270.16970458830923946RNA PCR Primer, Index 39 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA270.16970458830923946Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.16970458830923946No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.16341923318667503No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG250.15713387806411064RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA230.14456316781898176RNA PCR Primer, Index 3 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA220.13827781269641734RNA PCR Primer, Index 27 (96% over 30bp)
TCTCTGGGGCGGTCGCGTTGGCAGCGGATGCGGGAAGCCGGACTCTGGGC200.1257071024512885No Hit
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC200.1257071024512885No Hit
GTACATGGGGCTTCTCTGGGGCGGTCGCGTTGGCAGCGGATGCGGGAAGC190.11942174732872408No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA190.11942174732872408RNA PCR Primer, Index 26 (100% over 29bp)
GTACATGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCC180.11313639220615963No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA180.11313639220615963RNA PCR Primer, Index 31 (96% over 29bp)
GTGTATGATTATGCTGGGAAAAACAAAGTTCCAGAGCTACAAAAGTTTTT180.11313639220615963No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT180.11313639220615963RNA PCR Primer, Index 27 (96% over 28bp)
GTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTTCCTACAGACT160.1005656819610308No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT160.1005656819610308TruSeq Adapter, Index 1 (96% over 26bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT160.1005656819610308RNA PCR Primer, Index 31 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)