FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15910
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5463.4318038969201763No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT710.44626021370207414Illumina Single End PCR Primer 1 (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.2891263356379635No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC280.17598994343180388Illumina Single End PCR Primer 1 (95% over 21bp)
ATCATACACTGTGGAATCGGAGGTCAGTTTAGTTGGTGTGGCAAATATGA250.15713387806411064No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG240.15084852294154621No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT190.11942174732872408Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA180.11313639220615963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTATGATTATGCTGGGAAAAACAAAGTTCCAGAGCTACAAAAGTTTTT160.1005656819610308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)