FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001421495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405525
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA115200.4788975379594891No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC86270.35863273090073894No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC76220.3168539092297939No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC74550.3099115577680548No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA72180.3000592386277424No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC70580.29340788393386064No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT69170.2875463776098772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.2600263975639413No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC62300.2589871233930223No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC56610.2353332432629052No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG56400.2344602529593332No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT56270.23391983039045533No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC53660.2230698080460606No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA47960.1993743569491067No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC47730.1984182247118612No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA47520.19754523440828922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47350.1968385279720643No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT46200.19205786678583675No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA44940.18681992496440486No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT44660.18565593789297555No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT44310.18420095405368891No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG44310.18420095405368891No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT43530.18095841864042153No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC42890.17829787676286882No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG42770.17779902516082768No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG41740.17351721557664126No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC40710.16923540599245487No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT40560.16861184148990346No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA39020.16220991259704223No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC38630.16058864489040853No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA38560.1602976481225512No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA38520.16013136425520416No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG37950.15776181914550877No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT37540.15605740950520156No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG35960.1494891967449933No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA35450.1473690774363185No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA34570.1437108323546835No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT34290.14254684528325418No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC32990.1371426195944752No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA32390.13464836158426954No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG32340.13444050675008576No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31930.13273609710977854Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC31790.13215410357406387No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA31490.13090697456896105No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA31250.12990927136487876No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC30310.1260016004822232No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT29790.12383991020671163No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA29760.12371519730620134No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC29560.12288377796946612No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT29490.1225927812016088No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA29220.12147036509701625No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG29030.12068051672711778No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT28990.12051423285977074No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC28560.11872668128579No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA28440.11822782968374887No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC28050.11660656197711519No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA27150.11286517496180666No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT26840.11157647498986707No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG26760.11124390725517298No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT26730.11111919435466269No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC26610.11062034275262156No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT26400.10974735244904958No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC26340.10949792664802901No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT26250.10912378794649816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.10883279117864084No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT25690.10679581380363953No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA25690.10679581380363953No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC25630.10654638800261897No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG25620.10650481703578221No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG25130.10446783966078091No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG25040.10409370095925007No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT24990.10388584612506625No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA24290.10097587844649297No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT24120.10026917201026804No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)