FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001421512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854546
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA113510.6120635454715062No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC75060.4047351750778897No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC70760.3815489073875763No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA66020.35599009137546334No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC60510.32627931580020125No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC56610.30524991022061465No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC46320.24976463242216695No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC44470.23978914516005534No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC44360.23919600807960545No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA44080.2376862046020967No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT40700.21946071976645495No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT39560.2133136627508835No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT38500.2075979781574574No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG37650.2030146461721629No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA37110.20010288232268167No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC36850.19870092195070924No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT35770.1928773942517468No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA35550.19169112009084702No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC34540.1862450432612618No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC33490.18058328022060385No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG32910.17745583016004995No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA32560.17556857581316399No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT32500.17524504649655495No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG30770.16591661786766143No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT30160.162627403148803No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA29130.15707348321368142No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA29080.15680387544984054No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT28110.151573484831328No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG27960.15076466153980542No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC27820.15000975980105105No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA27320.1473136821626425No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC26370.1421911346496663No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA26340.14202936999136176No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC26340.14202936999136176No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG26310.14186760533305726No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC25960.1399803509861713No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG25820.1392254492474169No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA25410.1370146655839219No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT24860.1340489801816725No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC24100.12995094217129152No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA22830.12310290496973383No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC22640.12207839546713858No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT22350.12051467043686165No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA21640.11668624019032152No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA21220.11442153497405834No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA20790.112102908205027No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA20680.11150977112457713No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG20510.11059310472751821No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC20270.10929898746108212No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA20040.10805879174741419No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC19870.10714212535035529No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA19640.10590192963668736No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA19510.10520094945070113No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT19230.10369114597319237No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG19150.10325977355104698No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA18960.10223526404845175No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)