FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366561
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47081.2843701321198928No Hit
GAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAA11600.31645483289275184No Hit
ATCTAATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTAC10520.2869917967268749No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8200.22370083014832454No Hit
CTTCTATAGAATGAACATAGACATAACCCTGAAGCTTTTAGTTTACAGGG6680.1822343348037571No Hit
GTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCAC6030.164501951926146No Hit
CATTAGTTATTTCCAGACTCAAATAGATACACATTCAACCAATAAACTGA5560.15168007507618103No Hit
GCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCT5410.14758798671980924No Hit
GCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGCAAT5310.1448599278155614No Hit
ATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGGG5100.1391310041166409No Hit
GTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCG5090.1388581982262161No Hit
GTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGAT5070.13831258644536654No Hit
GACATAACCCTGAAGCTTTTAGTTTACAGGGAGTTTCCATGAAGCCACAA5070.13831258644536654No Hit
TTTCATAACTGTCCTTCCCACGGCTGTCCCAATCAGAAGGCGCGTTCAGG4860.132583662746446No Hit
CTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA4780.13040121562304774No Hit
GCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAA4770.13012840973262296No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT4300.117306532882658Illumina RNA PCR Primer (95% over 24bp)
GCTTGTGGCTGTGGGTTTCAGCACTCTGGTCATCCAGCTGACTCGTTTCA4230.1153968916496845No Hit
TCCCACGGCTGTCCCAATCAGAAGGCGCGTTCAGGTCCTGGGCAACGGGG3990.10884955027948963No Hit
GTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTT3900.10639429726566656No Hit
CTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATT3800.1036662383614187No Hit
AATCTGGACTGCTTGTGGCTGTGGGTTTCAGCACTCTGGTCATCCAGCTG3750.10230220890929476No Hit
CATTCAACCAATAAACTGAGAAAGAAGCATTTCATGTTCTCTTTCATTTT3730.1017565971284452No Hit
TGTATGCACCATTCAACTCCTCGCTTTCCATGTGTGAGGTGATGTCCTCG3720.10148379123802041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)