FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290838
Sequences flagged as poor quality0
Sequence length100
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT3746712.882429393683081No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT161685.559108507141432RNA PCR Primer, Index 26 (100% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151765.218025154897228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115353.9661254719121986No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT114103.9231462188572332RNA PCR Primer, Index 26 (100% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103653.5638396633177236No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46691.6053610601090642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39761.3670840811723366No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38491.3234171600684919No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG31051.0676046458853383RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG30011.0318459073436073RNA PCR Primer, Index 26 (96% over 27bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT24180.8313906710952489RNA PCR Primer, Index 26 (100% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC20490.7045159160769914RNA PCR Primer, Index 26 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.6085862232583087Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT17030.5855493436208473RNA PCR Primer, Index 26 (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.5270975594660945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.49133882092436343No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA10040.34520936053748136RNA PCR Primer, Index 26 (100% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8950.3077314518735516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC8920.3066999498002324RNA PCR Primer, Index 26 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC8620.2963849290670408No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG7640.26268919467194796No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7290.2506550038165577Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC7050.24240298723000434No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.2124894271037485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5830.2004552362483582No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.17432385039093928No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.16469649770662706No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4730.1626334935599887RNA PCR Primer, Index 26 (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4380.15059930270459843Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3760.12928159318933563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3740.1285939251404562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3390.11655973428506591No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3330.11449673013842758Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3320.11415289611398785Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT3120.1072762156251934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3040.10452554342967563No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2990.10280637330747702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2910.10005570111195924No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)