FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188368
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT614695.172555975926649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256732.160357734304525No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT242912.0440637916874236RNA PCR Primer, Index 27 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199331.6773423720598333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159521.3423451321476174No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT154371.2990083879740955RNA PCR Primer, Index 27 (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64780.545117337390438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63090.5308961533800978No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54030.4546571432418241No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG34020.28627495859868324RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC31250.2629656806645753No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.23065245782451227Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT26130.2198813835444913RNA PCR Primer, Index 27 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25590.2153373365826074No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.18470709409879768No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC12060.1014837154820729No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)