FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117012
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT620655.556341382187479No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321532.878482952734617No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT260432.331487933880746RNA PCR Primer, Index 39 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220311.9723154272290717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197371.7669461026381097No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT181411.6240649160438743RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG97910.8765348984612519RNA PCR Primer, Index 39 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70570.6317747705485707No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT66980.5996354560201681RNA PCR Primer, Index 39 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.575732400368125No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54620.4889831085073393No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG52370.46884008408146016Illumina PCR Primer Index 11 (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.28979097807364645Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC32170.28800048701356834No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.26185931753642755No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA28610.2561297461441775RNA PCR Primer, Index 39 (96% over 30bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27860.24941540466888448No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT27270.24413345604165398RNA PCR Primer, Index 39 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26800.23992580205047034No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC20390.18254056357496606Illumina PCR Primer Index 11 (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15600.1396583026860947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG13830.12381245680440317RNA PCR Primer, Index 27 (96% over 30bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13580.12157434297930551Clontech Universal Primer Mix Long (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA12530.11217426491389529No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG12090.10823518458172338RNA PCR Primer, Index 31 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11580.10366943237852413No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC11570.10357990782552022RNA PCR Primer, Index 39 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)