FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713417
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA257553.610090592178207No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31610.44307887252476463No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9690.13582519059680384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC9000.126153427798889No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT8760.12278933639091863Illumina Single End PCR Primer 1 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.1160611535749779No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC7980.11185603931501492No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7670.10751075457971986Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC7220.10120308318977539No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)