FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241754
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117944.878512868453056No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18170.7515904597235206No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT7720.3193328755677259Illumina Single End PCR Primer 1 (96% over 26bp)
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG5610.2320540714941635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC5020.20764909784326216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC4030.16669837934429213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC3990.16504380485948528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.1542890707082406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC3280.13567510775416333No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC3230.13360688964815473Illumina Single End PCR Primer 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC3210.1327796024057513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.11954300652729634No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2740.11333835220927058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTGGACAGGTCTCTTTCCTGGAATGCAGCCCAGGCACCTGTGCTCCT2420.10010175633081562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)