FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT3473.492702566683442RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT3253.271263210870659No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2322.33517866129844No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT2112.123804730749874RNA PCR Primer, Index 46 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1811.821841972823352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1561.5702063412179164No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG870.875691997986915RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG820.8253648716658278TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG710.7146451937594364RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG660.6643180674383493TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG580.58379466532461RNA PCR Primer, Index 26 (100% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.5334675390035228No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG500.5032712632108707RNA PCR Primer, Index 33 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.5032712632108707No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG450.45294413688978363TruSeq Adapter, Index 4 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.44287871162556625No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC390.3925515853044791No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT330.33215903371917466RNA PCR Primer, Index 46 (96% over 26bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG320.3220936084549572RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.3220936084549572Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT310.31202818319073977No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG310.31202818319073977TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA300.3019627579265224RNA PCR Primer, Index 27 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.291897332662305No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG280.28183190739808756RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA280.28183190739808756RNA PCR Primer, Index 31 (96% over 29bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA270.27176648213387017RNA PCR Primer, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA220.22143935581278312RNA PCR Primer, Index 3 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC210.21137393054856565No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG200.20130850528434827RNA PCR Primer, Index 23 (96% over 30bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG200.20130850528434827No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT180.18117765475591344TruSeq Adapter, Index 3 (96% over 26bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT180.18117765475591344RNA PCR Primer, Index 31 (96% over 27bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT170.17111222949169602TruSeq Adapter, Index 1 (96% over 26bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT170.17111222949169602RNA PCR Primer, Index 26 (100% over 27bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.17111222949169602No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT170.17111222949169602RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA140.14091595369904378RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA140.14091595369904378RNA PCR Primer, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA130.13085052843482636RNA PCR Primer, Index 33 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.12078510317060896No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA120.12078510317060896RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA110.11071967790639156RNA PCR Primer, Index 46 (96% over 28bp)
GTACATGGGCAGAGGGCTGATAGCCGAGAAAGTCTGAAGCCAGCCGCAAA110.11071967790639156No Hit
ATTCTAGACTCTGATAAACCTTATTTCTCCTAATTCTGTACAGCTCTACA110.11071967790639156No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA110.11071967790639156RNA PCR Primer, Index 39 (96% over 30bp)
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC100.10065425264217413TruSeq Adapter, Index 18 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100.10065425264217413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA100.10065425264217413TruSeq Adapter, Index 10 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)