FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3363.3819828887770504No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT180.18117765475591344Clontech Universal Primer Mix Long (96% over 26bp)
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT170.17111222949169602Illumina Single End PCR Primer 1 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.17111222949169602No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120.12078510317060896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC120.12078510317060896No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)