FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242729
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148246.107222457967527No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12660.5215693221658723No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT7340.3023948518718406Illumina Single End PCR Primer 1 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5680.23400582542671047Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5330.21958645238105046Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG4950.20393113307433391No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4730.1948675271599191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC4160.17138454819984428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC4010.1652048168945614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC3700.1524333721969769No Hit
GTACATGGGAGATGAAATAGTGAAAGAAGTGGAAGCCAAAGCACCAATTT3630.1495494975878449No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC3480.14336976628256204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC3400.1400739095864112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCC3150.12977435741093976No Hit
ATATTTATTGTGCCTGAGAGGCAAGGTGAGGGAAAAATCTCAACAGAAGC2470.10175957549365754No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)